Pac5.1/v5-his c
WebMay 8, 2003 · C. pipiens ace-1 encodes a putative 702-amino-acid protein, ... S2 cells were transfected with the recombinant pAc5.1/V5-His vector (Invitrogen) either alone (purple circles) or with expression of ... http://www.biofeng.com/adjy/zhili/RCASBP-AP-A-CC-37.html
Pac5.1/v5-his c
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WebFeb 26, 2007 · The coding sequence for the signal sequence of premembrane and E protein was cloned into the Drosophila expression vector pAc5.1/V5-His. A Drosophila cell line S2 was cotransfected with this construct as well as a … WebBriefly, the purified cloned vector (pAc5.1/V5-His/Kaz-S) was transfected by use of calcium phosphate (Invitrogen) into 2 × 10 6 to 4 × 10 6 S2 cells/ml in six-well cell culture plates (Costar). The cells were harvested at 48, 72, 96, 120, and 144 h after plasmid transfection.
WebpAc5.1/V5-His B: Other: Ampicillin: Invitrogen: pAc5.1/V5-His C: Other: Ampicillin: Invitrogen: Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. WebpAc5.1/V5-His A, B, or C in frame with the C-terminal peptide encoding the V5 epitope and the polyhistidine tag (if desired). “Cloning considerations” on page 8–“Multiple cloning site …
WebpAc5.1/V5-His A (Search Vector Database) Backbone manufacturer. ThermoFisher Scientific Backbone size w/o insert (bp) 5400 Total vector size (bp) 7400 Vector type. Insect Expression Growth in Bacteria. Bacterial Resistance(s) Ampicillin, 100 μg/mL ... http://www.labbase.net/Supply/SupplyItems-4229347.html
WebApr 22, 2024 · The PCR-amplified H2B-mCherry-HA was then inserted into the XhoI site of the Drosophila constitutive expression vector, pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy, the gypsy backbone used in previous publication [ 11 ] was amplified and cloned into the NotI/XbaI sites of pAc5 ...
WebpAc5.1 V5-His B. Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His A or pAc5.1/V5-His … bob\\u0027s delivery trackerWebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features: • Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency clive bridge closedWebpAc5.1/V5-His /LacZ PI-001 Insect expression plasmid-Drosophila Inquiry pAc5.1/V5-His A PI-002 Insect expression plasmid-Drosophila Inquiry pAc5.1/V5-His B PI-003 Insect expression plasmid-Drosophila Inquiry pAc5.1/V5-His C PI-004 Insect expression plasmid-Drosophila Inquiry pUAST PI-005 Insect expression plasmid-Drosophila Inquiry bob\u0027s deli west fallsWebGGA ACT G-3) for Listericin-V5-HIS, and forward (5-CCC GGG GAA TTC GAT AAT TCC CGC CAT GAA ACA GTA CCTGGTGC-3)andreverse(5-CCCGGG CTCGAGTTA TTTACGTCCCCAACTGGAACTG-3)forListericinwith-out tags and subcloned into EcoRI-XhoI sites of the pAC5.1-V5-HIS vector (Invitrogen). These vectors were transfected together … clive bridgeWebpAc5.1/V5-His /LacZ PVTY00728 2ug Plasmid type:Drosophila cell expression vectorPromoter:pAC5Cloning Method:Multiple cloning sites,restriction … clive bridgewaterWebRCASBP-AP (A) (CC#37) 价格： 1200元. 联系方式： I47-825O-882O. 相关技术服务： 质粒构建 基因合成 质粒大提. 立刻购买. clive branson fishingclive bridgham