Chloral hydrate anesthesia in mice

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August undefined, 2024

WebSep 30, 2013 · We demonstrate that 375 mg/kg 2,2,2-tribromoethanol followed after 5 min by 200mg/kg chloral hydrate provides an anesthesia time of 60 min, has negligible effects on ABR wave latencies and thresholds and non-systematic effects on amplitudes. ... Background: To examine psychoacoustics in mice, we have used 2,2,2-tribromoethanol … WebMay 1, 2024 · 1302 Purpose: To evaluate the effect of different concentrations of chloral hydrate and isoflurane anesthesia on brain uptake of 18F-FDG in mice. Methods: Total …

Increased anesthesia time using 2,2,2-tribromoethanol–chloral hydrate ...

WebProcedures: Sixty-minute dynamic [18 F] FDG PET scans were performed in adult male C57BL/6 mice anesthetized with isoflurane [control (in 100 % O 2), in medical air, in 100 % O 2 + insulin pre-treatment, and in 100 % O 2 after 18 h fasting], ketamine/xylazine, sevoflurane, and chloral hydrate. An additional group was scanned after awake uptake. WebFeb 17, 2009 · Anaesthesia was induced by chloral hydrate (500 mg kg −1, i.p.), and mice were killed 10 min after drug injection. Control mice received a PBS injection. Control mice received a PBS injection. Representative immunoblots are shown left to … hayward tyler limited

Chloral hydrate anesthesia and lens opacification in mice

WebAug 1, 2009 · Mice were anesthetized by i.p. injection of chloral hydrate (300 mg/kg), a well-known anesthetic, which is widely used despite some concern on its use for anesthesia of small animals (28). It does ... WebNov 20, 2013 · Mice were anesthetized with an intraperitoneal injection of a mixture of chloral hydrate and sodium pentobarbital (170 mg/kg chloral hydrate, 40 mg/kg sodium pentobarbital; 0.4 mL/100 g body weight) and mounted in a stereotaxic frame. WebAll surgery was performed under chloral hydrate anesthesia, and all efforts were made to minimize suffering. ... All mice were sacrificed under chloral hydrate anesthesia at day 21 after the first injection. Tumor nodules in the abdominal cavity were collected and weighed; mice weight and ascites were also evaluated in all groups. hayward \u0026 green aviation ltd

Mouse Anesthesia: The Art and Science - Oxford Academic

Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on …

WebChloral hydrate has been used in human and veterinary medicine as a sedative and hypnotic drug since the mid-19th century. Chloral hydrate's effects are primarily caused by its active metabolite trichloroethanol. Chloral hydrate is primarily a hypnotic and has little or no analgesic effect. In candidates for surgery, it is a satisfactory ... WebDec 1, 2013 · To explore effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice. Methods. The mice were anesthetized by isoflurane and ether inhalation, and also under intraperitoneal anesthesia with sodium pentobarbital and chloral hydrate. Blood was collected from the ventro-cardinal vein. hayward \u0026 scott exhaustWebPurpose: To study the effect of chloral hydrate in the eye lens in mice.Methods: We instilled tropicamide in the eyes of two groups of eight 129 strain wild-type mice and … hayward\u0027s cider farm

"WebJul 1, 1993 · Some studies (55,56) use 300-400 mg/kg chloral hydrate to perform surgery or establish animal models, the process of which requires deep anesthesia and long duration. ... " - Chloral hydrate anesthesia in mice

Chloral hydrate anesthesia in mice

A review of tribromoethanol anesthesia for production of …

WebJul 1, 2024 · Adjust the depth of anesthesia as dictated by changes in the monitored parameters to ensure continued surgical plane of anesthesia; Mice and Rats. ... WebMar 5, 2009 · For surgery and microinjection, chloral hydrate-anesthetized mice were positioned in a stereotaxic apparatus (NARISHIGE Scientific Instrument LAB); the lentivirus filled in the glass electrode was injected aiming at PAG (AP-4.5 mm; ML ±0 mm; DV 2mm). Lentivirus (1.5 μL) was microinjected into the PAG at a rate of 0.15 μL/min.

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WebHome Research A to Z WebJun 12, 1998 · Chloral hydrate is a hepatocarcinogen in mice but not rats. To examine this species-related difference, male and female B6C3F1 mice and Fischer (F344) rats were treated by gavage with 1 or 12 doses of chloral hydrate, and concentrations of the drug and its metabolites were determined in plasma at 0.25, 7, 3, 6, and 24 h and 2, 4, 8, and 16 d …

WebAbstract. Purpose: The aim of the present study was to study the time-course development of cold cataract in mice under general anesthesia. Methods: We anesthetized five groups of 10 mice (12 weeks old) with 400 mg/Kg intraperitoneal injections of chloral hydrate and exposed them to 0, 7, 15, 23, and 37°C for 1 hr. Cataract development was ... WebNov 22, 2024 · When primary cilia were removed using chloral hydrate, mineralization was inhibited. Although chloral hydrate is not a preferred method for primary cilia disruption now that more specific and effective tools are available, this result is not surprising and dental pulp cell mechanosensation warrants further exploration [ 58 ].

WebAll mice were intraperitoneally injected (10 mL·kg −1) with a 4% chloral hydrate solution for anesthesia. For ALI model group, the mice were instilled with 50 μL of LPS solution at a concentration of 1.5 mg·mL −1. (Dose was 3 mg·kg −1); the mice in the blank control group were instilled with the same volume of normal saline, and the ... WebThird, we admit that we neglect the potential problem of using chloral hydrate for euthanasia of rats. Chloral hydrate is a traditional anesthetic in animal experiments, and …

WebSep 30, 2013 · The mice used in this study were between 6 weeks and 4 months of age and were on a randomly mixed genetic background comprised of C57BL/6J and 129 Sv/Ev …

hayward\u0027s elite heating and cooling llcWebHepatic enzyme analysis: Chloral hydrate did not significantly induce either lauric acid 4-hydroxylase activity or CYP4A immunoreactive protein in any of the dosed groups of ad libitum-fed mice. However, 100 mg/kg did significantly induce both lauric acid 4-hydroxylase activity and CYP4A immunoreactive protein in the dietary-controlled mice. hayward\\u0027s elite heating and cooling llcWebLaboratory mice typically are anesthetized by either inhalation of volatile anesthetics or injection of drugs. Here we compared the acute and postanesthetic effects of combining both methods with standard inhalant monoanesthesia using sevoflurane in mice. After injection of fentanyl-midazolam or S-ketamine as premedication, a standard 50-min ... hayward\\u0027s ice creamWebmoethanol or fentanyl-fluanisone-diazepam anesthesia. However, a small but significant degree of radioprotection was observed under chloral hydrate or pentobarbital anes- ... (iii) Chloral hydrate: 2,2,2-trichloro-1,1-ethanediol (chloral hydrate by B.D.H. Chemicals Ltd.). The mice were boxed 5 min after an intraperitoneal injection of 300 mg/kg ... hayward\u0027s ice creamWebSynthesised by Justin Liebig in 1832 chloral hydrate is one of the oldest synthetic agents. Since 1869 it has been in use for hypnotic or sedative purposes. Chloral hydrate was used a lot from the end of the 19th century to the middle of the 20th century. Since then chloral hydrate has been less fre … hayward\u0027s ice cream menuWebSep 17, 2024 · Citation 20 – Citation 22 For determination of the baseline NE level, mice were treated to obtain sedation (chloral hydrate 300 mg/kg, i.p.), then blood was immediately collected by eyeball removal, and only the first two drops of blood were collected to avoid NE spillover. For measurement of the spleen NE level, homogenized … hayward\u0027s ice cream flavorsWebJun 28, 2024 · Much of our mouse anesthesia knowledge is derived from our anesthesia knowledge of humans and larger animals as the mechanisms are similar across species. … hayward\\u0027s game lodge camping